Year: 2017 | Month: December | Volume 7 | Issue 6
Allele Fixation in Prolactin and Pit-1 Genes Associated with Highest Fat Content than Milk Yield of Murrah Buffalo Population
Abstract:
Present study was focused on the genetic variability in Prolactin and Pituitary specific transcription factor-1 genes using Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP) and nucleotide sequencing techniques. DNA was isolated from 100 Murrah buffaloes maintained at directorate of livestock farms, GADVASU Ludhiana. A 294 bp fragment of PRL and 451 bp fragment of Pit-1 gene was amplified and digested using RsaI and HinfI restriction enzymes. The genotypes were then sequenced using sanger di-deoxy chain termination method and were compared with related species. Both RsaI and HinfI locus showed only one genotype. The amplicons of both genes exhibited absence of polymorphism with respect to restriction enzymes used and accordingly, the allelic frequency was found to be unity. The monomorphic pattern of the amplicon of these genes with respect to different enzymes indicated the concernedness of these genes. Percent diversity and divergence matrix analysis revealed that both genes are in close cluster with swamp buffalo and bos species as compared to other species included in the sequence comparison. The result suggested that the allele has been fixed in the herd and need to inclusion of the Murrah buffalo from other population in the herd to introduce desired genetic variability to enhance the milk production. Sequence comparison of PRL and Pit-1 genes of Murrah buffaloes with other species is little divergent.
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